Kisspeptin-10 10mg

Overview

Kisspeptins are a family of peptides encoded by the KISS1 gene, originally identified as metastasis-suppressor products and later recognized as the endogenous ligands of the G-protein-coupled receptor GPR54 (KISS1R). Kisspeptin-10 is the C-terminal decapeptide that retains full receptor activity and is the most widely used form in laboratory research.

Acu Aminos supplies Kisspeptin-10 as a 10 mg lyophilized powder for receptor-pharmacology and neuroendocrine-signaling studies.

Chemistry & specifications

Kisspeptin-10 is a C-terminally amidated decapeptide belonging to the RF-amide peptide family, sharing the Arg-Phe-NH₂ motif with related neuropeptides. The amidated C-terminus and the aromatic Phe residues are required for high-affinity binding to KISS1R.

Cellular structure & mechanism of action

KISS1R couples predominantly to Gα_q/11. Ligand binding activates phospholipase Cβ, generating IP₃ and diacylglycerol, releasing intracellular calcium and activating protein kinase C and the ERK1/2 MAPK cascade. In hypothalamic neuron models, kisspeptin signaling is a principal upstream regulator of GnRH neuron activity, and loss-of-function mutations in GPR54 were the discovery that linked this receptor to reproductive endocrinology. In cancer-cell models, KISS1/KISS1R signaling has been studied for its effects on cell motility and invasion.

Research applications

• KISS1R/GPR54 binding, calcium-mobilization and ERK-phosphorylation assays
• GnRH neuron and hypothalamic slice electrophysiology
• Cell-migration and metastasis-suppressor pathway research
• RF-amide peptide structure-activity studies

Selected references

1. Kotani M, Detheux M, Vandenbogaerde A, et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. J Biol Chem. 2001;276:34631–34636.
2. de Roux N, Genin E, Carel JC, et al. Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proc Natl Acad Sci USA. 2003;100:10972–10976.
3. Pinilla L, Aguilar E, Dieguez C, Millar RP, Tena-Sempere M. Kisspeptins and reproduction: physiological roles and regulatory mechanisms. Physiol Rev. 2012;92(3):1235–1316.

References are provided to help researchers locate primary literature. Acu Aminos makes no claims about the safety or efficacy of any compound in humans or animals.